FAM210B Knockout cell line (Hep G2)

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location: Home > Products > KO Cell Lines > FAM210B Knockout cell line (Hep G2)
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FAM210B Knockout cell line (Hep G2) Order

FAM210B Knockout cell line (Hep G2)
Catalog#
YKO-HT7471
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FAM210B Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FAM210B Knockout cell line (Hep G2)
Species
Human
Gene Name
FAM210B
Gene ID
116151
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript FAM210B-201)
Validation: PCR+Sanger Sequencing
FAM210B Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FAM210B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FAM210B gene knockout FAM210B Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
FAM210B Knockout cell line (Hep G2)
FAM210B Knockout cell line (Hep G2)
Catalog#
YKO-HT7471
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • FAM210B Knockout cell line (HEK293)

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    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • FAM210B Knockout cell line (HeLa)

    FAM210B Knockout cell line (HeLa)

    Organism: Human
    Gene Name: FAM210B
    Gene ID: 116151
    Size: 1*10^6
    Catalog#: YKO-XN47024
  • FAM210B Knockout cell line (HCT 116)

    FAM210B Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: FAM210B
    Gene ID: 116151
    Size: 1*10^6
    Catalog#: YKO-XN47023
  • FAM210B Knockout cell line (HEK293)

    FAM210B Knockout cell line (HEK293)

    Organism: Human
    Gene Name: FAM210B
    Gene ID: 116151
    Size: 1*10^6
    Catalog#: YKO-XN47022

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